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rabbit anti rhob  (Proteintech)


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    Structured Review

    Proteintech rabbit anti rhob
    Rabbit Anti Rhob, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+rhob/RHOB+Antibody/pmc12996707-337-10-15
    Average 93 stars, based on 36 article reviews
    rabbit anti rhob - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Membrane:

    Article Title: Epigenetic ALYREF/UHRF1/RHOB Axis in Corneal Wound Healing and Implications for Epithelial Tumorigenesis.
    Article Snippet: .. The separated proteins were transferred onto a nitrocellulose membrane and probed with primary antibodies-mouse antiUHRF1 (1:1000; Santa Cruz), rabbit anti-RHOB (1:1000; Proteintech), rabbit anti-Aly/Ref (1:1000; Abcam), or rabbit anti-β-actin (1:1000; Abcam) overnight at 4°C. .. After primary antibody incubation, the membranes were treated with IRDye 800CW fluorescent secondary antibody (1:5000; LICOR Biosciences).

    Article Title: Epigenetic ALYREF/UHRF1/RHOB Axis in Corneal Wound Healing and Implications for Epithelial Tumorigenesis
    Article Snippet: .. The separated proteins were transferred onto a nitrocellulose membrane and probed with primary antibodies-mouse anti-UHRF1 (1:1000; Santa Cruz), rabbit anti-RHOB (1:1000; Proteintech), rabbit anti-Aly/Ref (1:1000; Abcam), or rabbit anti-β-actin (1:1000; Abcam) overnight at 4°C. .. After primary antibody incubation, the membranes were treated with IRDye 800CW fluorescent secondary antibody (1:5000; LI-COR Biosciences).

    Blocking Assay:

    Article Title: Electromagnetic exposure changes human Schwann cell motility and transcriptomic profile of hearing-loss-related genes
    Article Snippet: An equal amount of proteins (20–30 μg) was separated through SDS-PAGE electrophoresis and transferred to a nitrocellulose membrane (GE Healthcare, Milan, Italy). .. After blocking, the membranes were exposed to primary antibodies, respectively: rabbit anti-RhoB (1:5000; # 14326-1-AP, Proteintech, Manchester, UK); mouse anti-RhoC (1:5000; # 67542-1-Ig, Proteintech); mouse anti-DPP4/CD26 (1:3000; #68383-1-Ig, Proteintech); mouse anti-alpha-tubulin (1:2000; T6199, Merk Life Science). .. Then, the membranes were incubated with HRP-conjugated anti-rabbit or anti-mouse secondary antibody for 1 h at room temperature and revealed using an enhanced chemiluminescence kit Cyanagen Ultra (Cyanagen, Bologna, Italy).

    Article Title: Electromagnetic exposure changes human Schwann cells motility and transcriptomic profile of hearing-loss related genes
    Article Snippet: An equal amount of proteins (20–30 μg) was separated through SDS-PAGE electrophoresis and transferred to a nitrocellulose membrane (GE Healthcare, Milan, Italy). .. After blocking, the membranes were exposed to primary antibodies, respectively: rabbit anti-RhoB (1:5000; # 14326-1-AP, Proteintech, Manchester, UK); mouse anti-RhoC (1:5000; # 67542-1-Ig, Proteintech); mouse anti-DPP4/CD26 (1:3000; #68383-1-Ig, Proteintech); mouse anti-alpha-tubulin (1:2000; T6199, Merk Life Science). .. Then, the membranes were incubated with HRP-conjugated anti-rabbit or anti-mouse secondary antibody for 1 h at room temperature and revealed using an enhanced chemiluminescence kit Cyanagen Ultra (Cyanagen, Bologna, Italy).

    other:

    Article Title: ATR/Chk1 signaling induces autophagy through sumoylated RhoB-mediated lysosomal translocation of TSC2 after DNA damage
    Article Snippet: Mouse anti-actin (1:2000, sc-47778), anti-Myc (1:2000, sc-40), anti-RhoB (1:1000, sc-8048), anti-GST (1:2000, sc-138), anti-UBC9 (1:2000, sc-271057), anti-LAMP1 (1:200, sc-20011), anti-tuberin (1:1000, sc-271314), rabbit anti-Chk1 (1:1000, sc-7898), and rabbit anti-RhoB (1:1000, sc-180) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA); mouse anti-HSP60 (1:200, H3524) and anti-FLAG (M2) (1:2000, F1804) antibodies were purchased from Sigma-Aldrich (St Louis, MO, USA); rabbit anti-TSC2 (1:1000, #4308), anti-ULK1 (1:1000, #8054), anti-AMPKα (1:1000, #2532), anti-PIAS1 (1:1000, #3550), anti-p70S6 kinase (1:1000, #2708), anti-phospho-p70S6 kinase (1:1000, #9205), anti-phospho-ULK1 (1:1000, Ser757#14202), anti-phospho-AMPKα (1:1000, Thr172#2531), anti-HSP60 (1:1000, #4870), anti-phospho-Threonine (1:1000, #9381), anti-Chk2 (1:1000, #2662), anti-Phospho-Chk1 (1:1000, ser345, #2348), and anti-Phospho-Chk2 (1:1000, Thr68, #2661) antibodies were purchased from Cell Signaling Technology; mouse anti-SQSTM1/P62 (1:1000, ab56416) antibody was purchased from Abcam; rat anti-HA (1:2000, #11867431001) monoclonal antibody was purchased from Roche (Mannheim, Germany); rabbit anti-LC3B/MAP1LC3B (1:1000, NB100-2220) was purchased from Novus; mouse anti-phosphoserine (1:1000, #05-1000), mouse anti-phospho-Histone H2A.X (Ser139)(1:200, #05-636) were purchased from Millipore; rabbit anti-RhoB (1:1000, 14326-1-AP) and rabbit anti-ATR (1:1000, 19787-1-AP) were purchased from Proteintech; inhibitors for Chk1 (#681637) and Chk2 (#220485) were purchased from Calbiochem; inhibitors for ATM CP-466722(#11002) and ATR VE-821(#14731) were purchased from MedChemExpress; chloroquine, methyl methanesulphonate, guanidine hydrochloride, and urea were purchased from Sigma-Aldrich (St Louis, MO, USA); Doxorubicin (HY-15142) and Campathecin (HY-16560) were purchased from MedChemExpress.



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    Image Search Results


    Construction and validation of machine-learning diagnostic models based on monocyte/macrophage-specific scDEGs. (A–D) Cross-validation performance surfaces illustrating model accuracy across tested hyperparameter combinations for Gradient Boosting Machine (A) , Support Vector Machine (B) , Random Forest (C) and Extreme Gradient Boosting (D) . (E–H) Feature-importance plots showing the top-ranked predictive genes identified by each algorithm—GBM (E) , SVM (F) , RF (G) , and XGBoost (H) . Importance values represent model-specific metrics: relative influence for GBM, permutation-based sensitivity for SVM, mean decrease in Gini impurity for RF, and gain across tree splits for XGBoost. (I–L) Precision–recall curves of the 4 machine-learning models evaluated on the validation set—GBM (I) , SVM (J) , RF (K) , and XGBoost (L, M) Upset plot showing the overlap of importance genes across the 4 algorithms (RHOB, TSPAN4, FRMD4B, CEBPB, CD4 and PDK4).

    Journal: Frontiers in Immunology

    Article Title: Immune cells with senescence-related transcriptional signatures orchestrate the inflammatory continuum in osteoarthritis synovium: a single-cell and machine learning study

    doi: 10.3389/fimmu.2026.1774722

    Figure Lengend Snippet: Construction and validation of machine-learning diagnostic models based on monocyte/macrophage-specific scDEGs. (A–D) Cross-validation performance surfaces illustrating model accuracy across tested hyperparameter combinations for Gradient Boosting Machine (A) , Support Vector Machine (B) , Random Forest (C) and Extreme Gradient Boosting (D) . (E–H) Feature-importance plots showing the top-ranked predictive genes identified by each algorithm—GBM (E) , SVM (F) , RF (G) , and XGBoost (H) . Importance values represent model-specific metrics: relative influence for GBM, permutation-based sensitivity for SVM, mean decrease in Gini impurity for RF, and gain across tree splits for XGBoost. (I–L) Precision–recall curves of the 4 machine-learning models evaluated on the validation set—GBM (I) , SVM (J) , RF (K) , and XGBoost (L, M) Upset plot showing the overlap of importance genes across the 4 algorithms (RHOB, TSPAN4, FRMD4B, CEBPB, CD4 and PDK4).

    Article Snippet: Membranes were blocked with Tris-buffered saline containing 0.1% Tween 20 (TBST) supplemented with 5% skim milk at room temperature for 1 h, followed by incubation with primary antibodies against RHOB (ABclonal, Cat. No. A22258), PDK4 (ABclonal, Cat. No. A13337), β-Tubulin (Santa Cruz Biotechnology, Cat. No. sc-5274), or GAPDH (Proteintech, Cat. No. 60004-1-Ig) at 4 °C overnight.

    Techniques: Biomarker Discovery, Diagnostic Assay, Plasmid Preparation

    Immunofluorescence and Western blot validation of RHOB and PDK4 expression in synovial macrophages. (A) Immunofluorescence images showing RHOB (red) colocalized with CD68 (green) in synovial tissue; nuclei are stained with DAPI (blue). (B) Quantification of RHOB/CD68 fluorescence intensity ratio. (C) Pixel-wise colocalization scatter plot of CD68 versus RHOB fluorescence signals (Pearson’s R = 0.80). (D) Representative immunofluorescence images showing PDK4 (red) colocalized with CD68 (green). (E) Quantification of PDK4/CD68 fluorescence intensity ratio. (F) Pixel-wise colocalization scatter plot of CD68 versus PDK4 fluorescence signals (Pearson’s R = 0.68). (G) Western blot analysis of RHOB and PDK4 protein expression in synovial tissues from OA patients and controls. CG, control group; OA, OA group. **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Journal: Frontiers in Immunology

    Article Title: Immune cells with senescence-related transcriptional signatures orchestrate the inflammatory continuum in osteoarthritis synovium: a single-cell and machine learning study

    doi: 10.3389/fimmu.2026.1774722

    Figure Lengend Snippet: Immunofluorescence and Western blot validation of RHOB and PDK4 expression in synovial macrophages. (A) Immunofluorescence images showing RHOB (red) colocalized with CD68 (green) in synovial tissue; nuclei are stained with DAPI (blue). (B) Quantification of RHOB/CD68 fluorescence intensity ratio. (C) Pixel-wise colocalization scatter plot of CD68 versus RHOB fluorescence signals (Pearson’s R = 0.80). (D) Representative immunofluorescence images showing PDK4 (red) colocalized with CD68 (green). (E) Quantification of PDK4/CD68 fluorescence intensity ratio. (F) Pixel-wise colocalization scatter plot of CD68 versus PDK4 fluorescence signals (Pearson’s R = 0.68). (G) Western blot analysis of RHOB and PDK4 protein expression in synovial tissues from OA patients and controls. CG, control group; OA, OA group. **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Article Snippet: Membranes were blocked with Tris-buffered saline containing 0.1% Tween 20 (TBST) supplemented with 5% skim milk at room temperature for 1 h, followed by incubation with primary antibodies against RHOB (ABclonal, Cat. No. A22258), PDK4 (ABclonal, Cat. No. A13337), β-Tubulin (Santa Cruz Biotechnology, Cat. No. sc-5274), or GAPDH (Proteintech, Cat. No. 60004-1-Ig) at 4 °C overnight.

    Techniques: Immunofluorescence, Western Blot, Biomarker Discovery, Expressing, Staining, Fluorescence, Control

    Drug–gene interaction mapping and molecular docking for RHOB and PDK4. (A) DGIdb-derived interaction network displaying the selected gene–compound pairs—PDK4 with SODIUM DICHLOROACETATE (DCA) and RHOB with CHEMBL1797159. The network also shows known disease associations of DCA. (B) Molecular docking pose of PDK4 with SODIUM DICHLOROACETATE (Vina score = −4.0 kcal/mol). (C) Molecular docking pose of RHOB with CHEMBL1797159 (Vina score = −5.2 kcal/mol).

    Journal: Frontiers in Immunology

    Article Title: Immune cells with senescence-related transcriptional signatures orchestrate the inflammatory continuum in osteoarthritis synovium: a single-cell and machine learning study

    doi: 10.3389/fimmu.2026.1774722

    Figure Lengend Snippet: Drug–gene interaction mapping and molecular docking for RHOB and PDK4. (A) DGIdb-derived interaction network displaying the selected gene–compound pairs—PDK4 with SODIUM DICHLOROACETATE (DCA) and RHOB with CHEMBL1797159. The network also shows known disease associations of DCA. (B) Molecular docking pose of PDK4 with SODIUM DICHLOROACETATE (Vina score = −4.0 kcal/mol). (C) Molecular docking pose of RHOB with CHEMBL1797159 (Vina score = −5.2 kcal/mol).

    Article Snippet: Membranes were blocked with Tris-buffered saline containing 0.1% Tween 20 (TBST) supplemented with 5% skim milk at room temperature for 1 h, followed by incubation with primary antibodies against RHOB (ABclonal, Cat. No. A22258), PDK4 (ABclonal, Cat. No. A13337), β-Tubulin (Santa Cruz Biotechnology, Cat. No. sc-5274), or GAPDH (Proteintech, Cat. No. 60004-1-Ig) at 4 °C overnight.

    Techniques: Derivative Assay

    GSEA results for optimal feature genes showed significant enrichment of PANoptosis pathway. ( A–C ) The results of single-gene GSEA for CEBPD. ( D – F ) The results of single-gene GSEA for EGR1. ( G – I ) The results of single-gene GSEA for HSPA1A. ( J – L ) The results of single-gene GSEA for HSPA1B. ( M – O ) The results of single-gene GSEA for RHOB.

    Journal: Journal of Inflammation Research

    Article Title: Unveiling PANoptosis in Acute Kidney Injury: An Integrative Multi-Dimensional Approach to Identify Key Biomarkers

    doi: 10.2147/JIR.S525222

    Figure Lengend Snippet: GSEA results for optimal feature genes showed significant enrichment of PANoptosis pathway. ( A–C ) The results of single-gene GSEA for CEBPD. ( D – F ) The results of single-gene GSEA for EGR1. ( G – I ) The results of single-gene GSEA for HSPA1A. ( J – L ) The results of single-gene GSEA for HSPA1B. ( M – O ) The results of single-gene GSEA for RHOB.

    Article Snippet: Primary antibodies against EGR1 (Proteintech, 22008-1-AP, 1:1000), CEBPD (Invitrogen, PA5-75232, 1:1000), HSPA1A (Invitrogen, PA5-34772, 1:5000), HSPA1B (Invitrogen, PA5-28369, 1:1000), and RHOB (CST, 63876S, 1:1000) were used.

    Techniques: